Contact Information

Preventive Medicine and Biometrics: Faculty

Uniformed Services University of the Health Sciences
Department of Preventive Medicine and Biometrics
4301 Jones Bridge Road
Bethesda, Maryland 20814-4799
Bldg. A, Rm. 2062
Phone: (301) 295-3173

Nicole L. Achee, Ph.D

Assistant Professor


Dr. Nicole L. Achee is a Ph.D. Medical Entomologist within the Department of Preventive Medicine, Division of Tropical Public Health at the Uniformed Services University. She has over 15yrs experience planning, managing, implementing, analyzing and publishing vector behavior research. This research has included habitat management strategies, experimental hut studies and evaluation of chemical actions against mosquito vectors in the international settings of Belize, Mexico, Peru, Thailand, Suriname, Indonesia and Africa. Dr. Achee also serves as adjunct faculty within the Department of Entomology at Katsetsart University in Bangkok, Thailand and advisor to students in the Emerging Infectious Disease Program at USUHS.


  • Ph.D., USUHS,
  • M.S., Texas A&M University,
  • B.S., St. Louis University


Main research interests are related to the epidemiology and control of arthropod-borne diseases including evaluation of vector ecology, management and control strategies, as well as disease risk modeling using GIS and remote sensing technologies. The principal investigator of a research program funded by the Bill & Melinda Gates Foundation focused on the development of a spatial repellent push-pull system to reduce mosquito vectors inside homes where they are most likely to feed on humans and transmit disease. She oversees a multi-national team of researchers in the USA, UK, Belize, Thailand, and Peru.


  • Malaria Epidemiology & Control
  • Introduction to GIS in Public Health
  • Modern Technology & Vector-borne Disease

Areas of Expertise

Selected Recent Publications

Paz-Soldan VA, Plasai V, Morrison AC, Rios-Lopez EJ, Guedez-Gonzales S, Grieco JP, Mundal K, Chareonviriyaphap T, Achee NL. 2011. Initial Assessment of the Acceptability of a Push-Pull Aedes aegypti Control Strategy in Iquitos, Peru and Kanchanaburi, Thailand. Am J Trop Med Hyg. 2011 84(2):208-217.

Chareonviriyaphap T, Grieco JP, Suwonkerd W, Prabaripai A, Polsomboon S, Thainchum K, Sungvornyothin S, Achee NL. 2010. An improved experimental hut design for the study of Aedes aegypti (Diptera: Culicidae) movement patterns in Thailand. J Vector Ecol. 35(2):428-31.

Masuoka P, Klein TA, Kim HC, Claborn DM, Achee N, Andre R, Chamberlin J, Small J, Anyamba A, Lee DK, Yi SH, Sardelis M, Ju YR, Grieco J. 2010. Modeling the distribution of Culex tritaeniorhynchus to predict Japanese encephalitis distribution in the Republic of Korea. Geospat Health. 5(1):45-57.

ongmee M, Nimmo D, Labb? G, Beech C, Grieco J, Alphey L, Achee N. 2010. Irritant and repellent behavioral responses of Aedes aegypti male populations developed for RIDL disease control strategies. J Med Entomol. 47(6):1092-8.

Moreno, M., Marinotti, O., Krzywinski, J., Tadei, W.P., James, A.A., Achee, N.L. and Conn, J.E. 2010. Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time. Malar. J. 9:127.

Thanispong, K., Achee, N.L., Bangs, M.J., Grieco, J.P., Suwonkerd, W., Prabaripai, A. and Chareonviriyaphap, T. 2009. Irritancy and repellency behavioral responses of three strains of Aedes aegypti exposed to DDT and alpha-cypermethrin. J. Med. Entomol. 46(6): 1407-1414.

Said, S.H., Grieco, J.P. and Achee, N.L. 2009. Evaluation of contact irritant and spatial repellent behavioral responses of male Aedes aegypti to vector control compounds. J. Am. Mosq. Control Assoc. 25(4): 436-431.

Suwannachote, N., Grieco, J.P., Achee, N.L., Suwonkerd, W., Wongtong, S. and Chareonviriyaphap, T. 2009. Effects of environmental conditions on the movement patterns of Aedes aegypti (Diptera: Culicidae) into and out of experimental huts in Thailand. J. Vector Ecol. 34(2): 267-275.

Dusfour, I., Achee, N.L., Roberts, D.R. and Grieco, J.P. 2009. Contact irritancy and spatial repellency behaviors in Anopheles albimanus Wiedemann (Diptera: Culicidae) collected in Orange Walk, Belize, C.A. J. Vector Ecol. 34(2): 232-237.

Mongkalangoon, P., Grieco, J.P., Achee, N.L., Suwonkerd, W. and Chareonviriyaphap, T. 2009. Irritability and repellency of synthetic pyrethroids on an Aedes aegypti population from Thailand. J. Vector Ecol. 34(2): 217-224.

Lawrence, K., Benante, J.P., Close, N. and Achee, N. 2009. Evaluation of efficacy and duration of the stick camouflage face paint with 30% Deet against mosquitoes in Belize. U.S. Army Medical Department Journal. 84-90.

Dusfour, I., Achee, N.L., Briceno, I., King, R. and Grieco, J.P. 2009. Comparative data on the insecticide resistance of Anopheles albimanus in relation to agricultural practices in northern Belize, CA. J. Pest. Sci. DOI: 10.1007/s10340-009-0268-7.

Dusfour, I., Achee, N.L., Sardelis, M.R., Chareonviriyaphap, T. and Grieco, J.P. 2009. Comparison of a novel high-throughput screening system with the bottle assay for evaluating insecticide toxicity. J. Pest. Sci. DOI: 10.1584/jpestics.G09-22.

Achee, N.L., Sardelis, M.R., Dusfour, I., Chauhan, K.R. and Grieco, J.P. 2009. Characterization of spatial repellent, contact irritant, and toxicant chemical actions of standard vector control compounds. J. Am. Mosq. Control Assoc. 25(2): 156-167.

McLean-Cooper N, Achee N.L., Foggie T, Grieco J and Williams J. 2008. Space optimizing methods for laboratory rearing of Aedes aegypti. J. Am. Mosq. Control Assoc. 24(3): 460-462.

Polsomboon, S., Grieco, J.P., Achee, N.L., Chauhan, K.R., Tanasinchayakul, S., Pothikasikorn, J. and Chareonviriyaphap, T. 2008. Behavioral responses of Catnip (Nepeta cataria) by two species of mosquitoes, Aedes aegypti and Anopheles harrisoni, in Thailand. J. Am. Mosq. Control Assoc. 24(4): 513-519.

Ayala-Sulca, Y.O., Ibarra-Juarez, L., Grieco, J.P., Achee, N., Mercado-Hernandez, R. and Fernandez-Salas, I. 2008. Behavioral response of Aedes aegypti (Linnaeus, 1762) exposed to Pyrethroid insecticides of frequent use in public health. Rev Peru Med Exp Salud Publica. 25(1): 26-34.

Mirabello, L., Vineis, J.H., Yanoviak, S.P., Scarpassa, V.M., Povoa, M.M., Padilla, N., Achee, N.L. and J.E. Conn. 2008. Microsatellite data suggest significant population structure and differentiation within the malaria vector Anopheles darlingi in Central and South America. J. BMC Ecol. 26;8:3.

Polsomboon, S., Poolprasert, P., Bangs, M.J., Suwonkerd, W., Grieco, J.P., Achee, N.L. and Chareonviriyaphap, T. 2008. Effects of Physiological Conditioning on Behavioral Avoidance using a Single Age Group of Aedes aegypti (L.) Exposed to Deltamethrin and DDT. J Med Entomol. 45(2):251-9.

Achee, N.L., Grieco, J.P., Andre, R.G., Roberts, D.R. and Rejmankova, E. 2007. A mark-release-recapture study to define the flight behavior of Anopheles vestitipennis and An. albimanus in Belize, Central America. J. Am. Mosq. Control Assoc. 23: 276-282.

Grieco, J.P., Achee, N.L., Chareonviriyaphap, T., Suwonkerd, W., Chauhan, K., Sardelis, M., and Roberts, D.R. 2007. A new classification system for the actions of IRS chemicals traditionally used for malaria control. PlosOne. 2(1): e716.

Grieco, J.P., E. Rejm?nkov?, N.L. Achee, C.N. Klein, R. Andre and D. Roberts. 2007. Habitat suitability for three species of Anopheles mosquitoes: Larval growth and survival in reciprocal placement experiments. J. Vector Ecol. 32(2): 176-87.

Achee, N.L., Grieco, J., Rejmankova, E. and Roberts, D.R. 2006. A delayed-release mechanism for mark-release-recapture studies. J. Am. Mosq. Control Assoc. 22: 573575.

Achee, N.L., Grieco, J.P., Andre, R.G., Roberts, D.R. and Rejmankova, E. 2006. The use of remote sensing and GIS to predict locations of Anopheles darlingi positive breeding sites within the Sibun River in Belize, Central America. J. Med. Entomol. 43(2): 382-392.

Achee, N.L., Grieco, J.P., Andre, R.G., Roberts, D.R. and Rejmankova, E. 2006. Biting patterns and seasonal densities of Anopheles mosquitoes in Cayo District, Belize, Central America with emphasis on Anopheles darlingi J. Vector Ecol. 31(1): 45-57.

Achee, N.L., Grieco, J.P., Andre, R.G., Roberts, D.R. and Rejmankova, E. 2006. Experimental evaluation of overhanging bamboo in Anopheles darlingi larval habitat selection in Belize, Central America. J. Vector Ecol. 31(1): 145-51.

Suwonkerd, W., P. Mongkalangoon, A. Parbaripai, J. Grieco, N. Achee, D. Roberts and T. Chareonviriyaphap. 2006. The effect of host type on movement patterns of Aedes aegypti (Diptera:Culicidae) into and out of experimental huts in Thailand. J. Vector Ecol. 31(2): 311-318.

Grieco, J.P., Johnson, S., Achee, N.L., Masuoka, P., Pope, K., Rejmankova, E., Vanzie, E., Andre, R.G., and Roberts, D. 2006. Distribution of Anopheles albimanus, Anopheles vestitipennis and Anopheles crucians associated with land use in Northern Belize. J. Med. Entomol. 43(3): 614-622.

Achee, N.L., Grieco, J.P., Andre, R.G., Roberts, D.R. and Rejmankova, E. 2005. A mark-release-recapture study utilizing a novel portable hut design to define the flight behavior of Anopheles darlingi in Belize, Central America. J. Am. Mosq. Control Accoc. 21(4): 366-379.

Rejmankov E, Grieco J, Achee N, Masuoka P, Pope K, Roberts D, and Richard M.Higashi: 2005. Freshwater community interactions and malaria. In: Disease ecology community structure and pathogen dynamics. Eds: S. Collinge and C. Ray. pp. 240.

Grieco, J.P., Achee, N.L., Sardelis, M., Chauhan, K. and Roberts, D.R. 2005. A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals. J. Am. Mosq. Control Assoc. 21(4): 404-411.

Grieco, J.P., Vogtsberger, R.C., Achee, N.L., Macek, P., Briceno, I., King, R., Andre, R.G., Roberts, D.R., and Rejmankova, E. 2005. Evaluation of habitat management strategies for the reduction of malaria vectors in Northern Belize. J. Vector Ecol. 30(2): 235-243.

Chareonviriyaphap, T., W. Suwonderd, Mongkalangoon, P., Achee, N., Grieco, J. and Roberts, D. 2005. The use of an experimental hut for evaluating the entering and exiting behavior of Aedes aegypti (Diptera: Culicidae), a primary vector of dengue in Thailand. J. Vector Ecol.30(2): 344-346.

Grieco, J.P., Achee, N.L., Andre, R.G. and Roberts, D.R. 2005. Comparative susceptibility of three species of Anopheles from Belize, Central America to Plasmodium falciparum (NF-54). J. Am. Mosq. Control Assoc.21(3): 279-290

Rejmankova E, Hagashi R, Grieco J, Achee, N. L. and Roberts D. 2005. Volatile substances from larval habitats mediate species-specific oviposition in Anopheles mosquitoes. J. Med. Entomol. 42(2):95-103.

Santos,P.D., Achee, N.L., Quinnan, G.V. and Roberts, D.R. 2004. Use of polymerase chain reaction technique to confirm VecTest screening results in Plasmodium faliciparum and P. vivax VK210 laboratory infected Anopheles stephensi mosquitoes. J. Am. Mosq. Control Assoc. 20(3): 265-71.

Grieco, J.P., Achee, N.L., Briceno, I., King, R., Andre, R.G. Roberts, D.R. and Rejmankova E. 2003 Comparison of life table attributes from newly established colonies of Anopheles albimanus and Anopheles vestitipennis in northern Belize. J. Vector Ecol. 28(2): 200-7.

Grieco, J.P., Achee, N.L., Andre, R.G. and Roberts, D.R. 2002. Host feeding preferences of Anopheles species collected by manual aspiration, mechanical aspiration, and from a vehicle-mounted trap in the Toledo District, Belize, Central America. J. Am. Mosq. Control Assoc. Dec;18(4): 307-15.

Achee, N.L., Korves, C.T., Bangs, M.J., Rejmankova, E., Lege, M., Curtin, D., Lenares, H., Alonzo, Y., Andre, R.G. and Roberts, D.R. 2000. Plasmodium vivax polymorphs and Plasmodium falciparum circumsporozoite proteins in Anopheles (Diptera: Culicidae) from Belize, C.A. J. Vector Ecology 25(2): 203-211.

Grieco, J.P., Achee, N.L., Andre, R.G. and Roberts, D.R. 2000. A comparison study of house entering and exiting behavior of Anopheles vestitipennis (Diptera: Culicidae) using experimental huts sprayed with DDT or deltamethrin in the southern District of Toledo, Belize, C.A. J. Vector Ecology 25 (1): 62-73.

Professional Activities

  • Member: Armed Forces Pest Management Board (AFPMB) Scientific Research Committee
  • Member: World Health Organization Pesticide Evaluation Scheme (WHOPES), Global Partnership for the Development of Pesticides for Public Health (GCDPP)
  • Working Group Member: DMID, NIAID, NIH, Bethesda, MD USA. Development of guidelines for the ethical consideration of human volunteers in vector biology research.
  • Steering Committee Member/Working Group Leader Bill & Melinda Gates Foundation. Vector control consultation to establish an R&D agenda for spatial repellent vector control products designed to eliminate and/or eradicate malaria.
  • AAAS
  • American Society of Tropical Medicine & Hygiene
  • American Mosquito Control Association
  • Entomological Society of America
  • Society of Vector Ecology
  • Center for Application of Remote Sensing and GIS in Public Health