Department of Pathology: Faculty
Uniformed Services University of the Health Sciences
Department of Pathology
4301 Jones Bridge Road, C1094
Bethesda, Maryland 20814-4799
Phone: (301) 295-3450
fax: (301) 295-1640
Michael J. Daly, Ph.D.
Queen Mary, University of London, UK, Ph.D., 1994
Current Research Interests:
Bacteria belonging to the family Deinococcaceae are some of the most radiation-resistant organisms yet discovered. Deinococcus radiodurans was first reported in 1956 by Andy Anderson and coworkers of the Oregon Agricultural Experimental Station, Corvalis, Oregon. The genes and cellular pathways underlying the survival strategies of D. radiodurans have been under investigation ever since, and its resistance characteristics are being exploited in the development of bioremediation processes for cleanup of highly radioactive US Department of Energy waste sites. One original goal of radiobiology was to explain why cells are so sensitive to ionizing radiation (IR). Early studies in bacteria incriminated DNA as the principal radiosensitive target, an assertion that remains central to modern radiation toxicity models. More recently, the emphasis has shifted to understanding why bacteria such as D. radiodurans are extremely resistant to IR. Unfortunately, as key features of DNA-centric hypotheses of extreme resistance have grown weaker, the study of alternative cellular targets has lagged far behind, mostly because of their relative biological complexity. Recent studies have shown that extreme levels of bacterial IR resistance correlate with high intracellular Mn(II) concentrations, and resistant and sensitive bacteria are equally susceptible to IR-induced DNA damage. Our most recent work has established a mechanistic link between Mn(II) and protection of proteins from radiation damage. In contrast to resistant bacteria, naturally sensitive bacteria are highly susceptible to IR-induced protein oxidation. We have proposed that sensitive bacteria sustain lethal levels of protein damage at radiation doses that elicit relatively little DNA damage, and that extreme resistance in bacteria is dependent on protein protection.
Representative Publications: 1994-2006
H. Brim, J. P. Osborne, H. M. Kostandarithes, J. K. Fredrickson, L. P. Wackett and MICHAEL J. DALY (2006) Deinococcus radiodurans engineered for complete toluene degradation facilitates Cr(VI) reduction. Microbiology, Aug;152(Pt 8):2469-77.
X. Qiu, MICHAEL J. DALY, A. Vasilenko, M. V. Omelchenko, L. Wu, J. Zhou, G. W. Sundin and J. M. Tiedje (2006) Transcriptome analysis applied to survival of Shewanella oneidensis MR-1 exposed to ionizing radiation. J. Bacteriol., 188, 1199-1204.
J. W. Szostak, R. E. Blake, MICHAEL J. DALY, D. H. Grinspoon, A. D. Keefe and G. J. Olsen (2006) Assessment of Planetary Protection Requirements for Venus Missions. National Academy Press, Commission on Physical Sciences, Mathematics, and Applications.
J. Qin, L. Song, Hassan Brim, MICHAEL J. DALY and A. O. Summers (2006) Sequestration and protection by the MerR metal binding domain (MBD). Microbiology, 152, 709-719.
MICHAEL J. DALY (2006) Modulating radiation resistance: Insights based on defenses against reactive oxygen species (ROS) in the radioresistant bacterium Deinococcus radiodurans. Clin. Lab. Med. 26, issue 2, In Press.
M. V. Omelchenko, Y. I. Wolf, E. K. Gaidamakova, V. Y. Matrosova, A. Vasilenko, MICHAEL J. DALY, E. V. Koonin and K. S. Makarova (2005) Comparative genomics of Thermus thermophilus HB27 and Deinococcus radiodurans R1: Divergent paths of adaptation to thermophily and radiation resistance. BMC Evolutionary Biology, 5, 57-79.
D. Ghosal, M. V. Omelchenko, E. K. Gaidamakova, V. Y. Matrosova, A. Vasilenko, A. Venkateswaran, H. M. Kostandarithes, H. Brim, K. S. Makarova, L. P. Wackett, J. K. Fredrickson and MICHAEL J. DALY (2005) How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiology Reviews 29, 361-375.
J. Szostak, J. C. Wheeler, S. A. Benner, J. Berry, R. Blake, W. Calvin, MICHAEL J. DALY, K. H. Freeman, J. P. Gogarten, J. F. Kasting, A. Keefe, M. Keller, S. Pizzarello, J. Siefert, R. Summons, N. Woolf and L. M. Ziurys (2005) The astrophysical context of life. The National Academies Press, National Academy of Sciences, Washington, D.C,
MICHAEL J. DALY, E. K. Gaidamakova, V. Y. Matrosova, A. Vasilenko, M. Zhai, A. Venkateswaran, M. Hess, M. V. Omelchenko, H. M. Kostandarithes, K. S. Makarova, L. P. Wackett, J. K. Fredrickson and D. Ghosal (2004) Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance. Science 306, 925-1084.
J. K. Fredrickson, J. M. Zachara, D. L. Balkwill, D. Kennedy, S. W. Li, H. M. Kostandarithes, MICHAEL J. DALY, M. F. Romine and F. J. Brockman (2004) Geomicrobiology of high level nuclear waste contaminated vadose sediments at the Hanford Site, Washington state. Appl. Environ. Microbiol. 70, 4230-4241.
M. Sandigursky, S. Sandigursky, P. Sonati, MICHAEL J. DALY and W. A. Franklin (2004) Multiple uracil-DNA glycosylase activities in D. radiodurans. DNA Repair, 3, 163-169.
J. R. Battista, M. M. Cox, MICHAEL J. DALY, I. Narumi, M. Radman and S. Sommer (2003) The structure of Deinococcus radiodurans. Science, 302, 567-568.
H. Brim, A. Venkateswaran, H. M. Kostandarithes, J. K. Fredrickson and MICHAEL J. DALY (2003) Genetic development of D. geothermalis for bioremediation of high temperature radioactive waste environments. Appl. Environ. Microbiol., 69, 4575-4582.
Y. Liu, J. Zhou, A. Beliaev, J. Stair, L. Wu, D.K. Thompson, D. Xu, A. Venkateswaran, M. Omelchenko, M. Zhai, E. K. Gaidamakova, K. S. Makarova, E. Koonin and MICHAEL J. DALY (2003) Transcriptome dynamics of Deinococcus radiodurans recovering from ionizing radiation. Proc. Natl. Acad. Sci. USA, 100, 4191-4196.
M. S. Lipton, L. Pasa-Tolic, G. A. Anderson, D. J. Anderson, D. Auberry, J. R. Battista, MICHAEL J. DALY, J. K. Fredrickson, K. K. Hixson, H. Kostandarithes, T. Conrads, C. Masselon, M. Markille, R. J. Moore, M. F. Romine, Y. Shen, N. Tolic, H. R. Udseth, T. D.Veenstra, A. Venkateswaran , K. K Wong, R. Zhao and R. D. Smith (2002) Global analysis of the Deinococcus radiodurans R1 proteome using accurate mass tags. Proc. Natl. Acad. Sci. USA. 99, 11049-11054.
J-I Kim, A. K. Sharma, S. N. Abbot, E. A Wood, D. Dwyer, A. Jambura, K. W. Minton, R B. Inman, MICHAEL J. DALY and M. M. Cox (2002) RecA protein from the extremely radioresistant bacterium Deinococcus radiodurans: Expression, purification, and characterization.
MICHAEL J. DALY (2002) The Quarantine and certification of martian samples: Appendix A: Deinococcus radiodurans as an analogue to extremophile organisms that may have survived on Mars. National Academy Press, Commission on Physical Sciences, Mathematics, and Applications, pp. 67-69
K. S. Makarova, L. Aravind, Y. I. Wolf, R. L.Tatusov, K. Minton, E. V. Koonin and MICHAEL J. DALY (2001) The genome of the extremely radiation resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiology and Molecular Biology Reviews 65, 44-79.
MICHAEL J. DALY (2001) The emerging impact of genomics on the development of biological weapons: Threats and benefits posed by engineered extremophiles. Clinics in Laboratory Medicine 21, 619-629.
J. Nolling, G. Breton, M. V. Omelchenko, K. S. Makarova, Q. Zeng, R., Gibson, H. M. Lee, J. Dubois., D. Qiu, J. Hitti, Y. I. Wolf, R. L., Tatusov, F. Sabathe, L. Doucette-Stamm, R. L. Tatusov, P. Soucaille, MICHAEL J. DALY, G. N. Bennett, E. V. Koonin and D. R. Smith (2001) Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J. Bacteriol. 183, 4823-4838.
A. F. Cheng, B. C. Clark, MICHAEL J. DALY, E. I. Friedman, B. M. Jakosky, R. Y Morita, A.-L. Reysenbach, D. A. Stahl and L. W. Esposito (2001) Planetary Protection for Europa, Space Studies Board, National Research Council, National Academy of Sciences, Washington, D.C.
MICHAEL J. DALY (2000) Engineering radiation-resistant bacteria for environmental biotechnology. Current Opinion in Biotechnology 11, 280-285.
H. Brim, S. McFarlan, L. Wackett, K. W. Minton, M. Zhai, J. Fredrickson and MICHAEL J. DALY (2000) Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments. Nature Biotechnology 18, 85-90.
J. K. Fredrickson, H. M. Kostandarithes, A. W. Li, A. E. Pyle and MICHAEL J. DALY (2000) Reduction of Fe(III), Cr(VI), U(VI), and Tc(VII) by Deinococcus radiodurans. Appl. Environ. Microbiol. 66, 2006-2011.
A.Venkateswaran, S. C. McFarlan, D. Ghosal, K. W. Minton, A. Vasilenko, K. Makarova, L. P. Wackett and MICHAEL J. DALY (2000) Physiologic determinants of radiation resistance in Deinococcus radiodurans. Appl. Environ. Mircobiol. 66, 2620-2626.
K. S. Makarova, L. Aravind, MICHAEL J. DALY and E. V. Koonin (2000) Specific expansion of protein families in the radioresistant bacterium Deinococcus radiodurans. Genetica 108, 25-34.
O. White, J. A. Eisen, J. F. Heidelberg, E. K. Hickey, J.D. Peterson, R. J. Dodson, C. Zalewski, K. S. Makarova, L. Aravind, MICHAEL J. DALY, K. W. Minton, R. D. Fleischmann, K. A. Ketchum, K. E. Nelson, S. Salzberg, H. O. Smith, J. C. Venter, C. M. Fraser et al., (1999) Sequencing and functional analysis of the Deinococcus radiodurans genome. Science 286, 1571-1577.
J. Lin, R. Qi, C. Aston, J. Jing, T. S. Anantharaman, B. Mishra, O. White, K. W. Minton, MICHAEL J. DALY, J. C. Venter and D. C. Schwarzt (1999) Whole genome shotgun optical mapping of Deinococcus radiodurans using genomic DNA molecules. Science 285, 1558-1561.
R. C. Richmond, R Sridhar and MICHAEL J. DALY (1999) Physicochemical survival pattern for the radiophile Deinococcus radiodurans: A polyextremophile model for life on Mars. SPIE, 3755, 210-222.
K. S. Makarova, Y. I. Wolf, K. W. Minton, O. White and MICHAEL J. DALY (1999) Short repeats and insertional elements in Deinococcus radiodurans and comparison to other bacterial species. Res. Microbiol. 150, 711-724.
C. Lange, L. P. Wackett, K. W. Minton and MICHAEL J. DALY (1998) Engineering a recombinant Deinococcus radiodurans for organopollutant degradation in radioactive mixed waste environments. Nature Biotechnology 16, 929-933.
MICHAEL J. DALY and K. W. Minton (1997) Recombination between a resident plasmid and the chromosome following irradiation of the radioresistant bacterium Deinococcus radiodurans. Gene 187, 225-229.
J. D. Carroll, MICHAEL J. DALY and K. W. Minton (1996) Expression of recA in Deinococcus radiodurans. J. Bacteriol. 178, 130-135.
MICHAEL J. DALY and K. W. Minton (1996) An alternative pathway for recombination of chromosomal fragments precedes recA-dependent recombination in the radioresistant bacterium Deinococcus radiodurans. J. Bacteriol. 178, 4461-4471.
MICHAEL J. DALY and K. W. Minton (1995) Resistance to radiation. Science 270, 1318.
K. W. Minton and MICHAEL J. DALY (1995) A model for repair of radiation induced DNA DSBs in the extreme radiophile Deinococcus radiodurans. Bioessays 17, 457-464.
MICHAEL J. DALY and K. W. Minton (1995) Interchromosomal recombination in the extremely radioresistant bacterium Deinococcus radiodurans. J. Bacteriol. 177, 5495-5505.
MICHAEL J. DALY, L. Ouyang, P. Fuchs and K. W. Minton (1994) In vivo damage and recA-dependent repair of plasmid and chromosomal DNA in the radioresistant bacterium Deinococcus radiodurans. J. Bacteriol. 176, 3508-3517.
MICHAEL J. DALY, L. Ouyang and K. W. Minton (1994) Interplasmidic recombination following irradiaition of the radioresistant bacterium Deinococcus radiodurans. J. Bacteriol. 176, 7506-7515.