Quick Resource Links
Biomedical Instrumentation Center
Uniformed Services University of the Health Sciences
4301 Jones Bridge Road
Bethesda, Maryland 20814-4799
BIC is located on the ground floor of B building in rooms G230 - G242.
Take the freight elevator in B bldg down to the ground floor
Make a left at the exercise room and go to the end of the hall
Make a right and BIC is halfway down the hall on your left
Microscopic Imaging: Instruments
Leica AF6000 Time-lapse Imaging System
The Leica AF6000 consists of a Leica inverted microscope equipped with a fully motorized 3-axis stage and CO2 and temperature control. Combined with the Leica AFS software package, the AF6000 is ideally suited for general fluorescence or transmitted light imaging. Potential applications include: (1) fluorescence imaging in blue, green, red, and far-red of cells or tissues in plates or on glass slides; (2) time-lapse imaging of live cells with fluorescence, transmitted light (brightfield or DIC), or both. The Leica Automatic Focus Control as well as software-based autofocus routines ensures that the specimen remains in focus during long experiments; (3)image tiling. The Leica AF6000 can collect multiple overlapping images of a large specimen and then stitch the images together to create a single image that provides both wide-field coverage and the ability to zoom in and see fine details; (4) deconvolution is an image processing method in which out of focus light which degrades image quality and resolution is removed. The integrated deconvolution package makes optimizing your images easy.
Leica UC6 ultramicrotome with FC6 cryo attachment
The Leica UC6 is a state of the art ultramicrotome allowing users to obtain high quality sections (50 nanometers - 1 micrometer thick) of properly embedded specimens. The cryo attachment uses liquid nitrogen to keep knives and specimens at ultra-cold temperatures so that unembedded frozen material may be sectioned.
Multi-photon Confocal Electrophysiology System
The multi-photon confocal electrophysiology system consists of a Zeiss AxioExaminer.Z1 upright microscope equipped with a direct-coupled Coherent Chameleon tunable infrared laser. The combination of the infrared laser, non-descanned detectors (currently green and red emission detection), and a range of long working distance water immersion objective lenses (10, 20 and 40x) allows for the deepest imaging possible in tissue slices or whole animal studies. The system is equipped with an Axon Multiclamp 700B amplifier and two headstages, mounted on motorized micromanipulators (Scientifica). A GRASS S88 dual output square pulse stimulator, a nano injector, and recording chamber perfusion and temperature control are also available. Electrophysiology components provide measurements of electrical activity and the ability for direct electrical stimulation in the tissue under 2-photon observation.
The stereology system is composed of a Zeiss AxioImager.M2 upright microscope and MicroBrightField's Stereo Investigator software package. The microscope is equipped with a wide range of objective lenses (2.5x - 63x), standard and oil immersion condensers, an X-Cite fluorescent light source, and filter cubes appropriate for imaging red, green, and blue fluorophores. The system also has a motorized stage and two digital cameras. The Zeiss Axiocam MRc is a color camera suitable for brightfield imaging, while the Hamamatsu Orca-R2 captures monochrome fluorescence images.
The Stereo Investigator software gives investigators image analysis, mapping, and measurement tools all in one software package, and allows for unbiased quantification of such parameters as cell numbers, lengths, areas, and volumes. All formulas and references used for calculations are provided with the software so that researchers are aware of exactly how the quantification is performed. The software also implements several statistical analysis methods so that users may have confidence in their results. More details about the Stereo Investigator software package can be found at: http://www.mbfbioscience.com/stereo-investigator
Zeiss 710 Confocal Laser Scanning Microscope
The Zeiss 710 confocal laser scanning microscope consists of a Zeiss AxioImager inverted microscope equipped with four visible lasers as well as a tunable infrared laser for multi-photon excitation. This wide selection of excitation wavelengths coupled with user selectable emission wavelength ranges means that researchers are free to use almost any fluorescent molecule when designing their experiments. The system configuration allows researchers to collect images of up to 3 fluorescent channels and a transmitted light image simultaneously. The multi-photon excitation provided by the IR laser is ideal for deep imaging within tissues and also for FRET or uncaging studies in which the researcher needs to ensure that excitation and activation is limited to a single focal plane. The Zeiss 710 is also equipped with an environmental chamber which allows for the control of temperature, humidity and CO2 levels, making long-term live cell imaging possible.
Zeiss Pascal Confocal Microscope
The Zeiss Pascal inverted confocal microscope has three visible lasers and filter sets appropriate for imaging most standard blue, green, and red fluorescent stains. The Pascal can collect two fluorescent channels as well as one transmitted light channel simultaneously and is equipped with objectives ranging from 10x - 100x. A live cell observation system allows for the control of CO2 and temperature as well as perfusion of fresh media when cells are cultured on compatible cover-slips.